Updated 2026-10-07 · 2 certificates ·
Issued by AxisPharm, LLC · Laboratory documentation · Research use only
This guide covers two of the certificates of analysis we publish for NAD+ (nicotinamide
adenine dinucleotide), one for each size in the catalog. They were issued by AxisPharm, LLC, the testing laboratory our
supplier, Protixa, sends samples to, and Protixa provided them to us. NAD+ is a dinucleotide cofactor, not a peptide;
its certificates use the same LC-MS and endotoxin format as the peptide reports. Below: every field each certificate
prints, what each test measures, how to check one, what they leave out and their caveats. The main caveat: the
1000 mg certificate's ID is dated two days after its report date. That certificate's QR code opens the laboratory's own
copy, and when we compared the two files they were identical.
We also publish the certificate for lot NAD500VAS0126
on the product page and in the certificate library. Its PDF shows a change made after the report date (the best-before date was retyped three days later), so this guide doesn't go through it field by field.
Issued by AxisPharm, LLC, provided by our supplier, Protixa. Identity for every lot was by LC-MS;
the detected masses are under identity below.
Each certificate, field by field
The fields below are copied from the PDF as printed, including any that disagree with each other.
NAD+ 500mg · Lot NAD500VAS0626
Laboratory (From)
AxisPharm, LLC
Addressed to (To)
Protixa
Brand name
Protixa
Certificate ID
13Jul2026-01
Report date
July 13, 2026
Sample received
07/07/2026
Condition on receipt
Acceptable
Sample name
NAD+, 500mg
Method(s)
LCMS and LAL Endotoxin Assay
Net weight measured
503.5 mg
Molecular weight
663.43 g/mol
Detected
664.8 [M+H]
UV purity
>99.2% (no wavelength in the table; the UV trace is labelled TWC, all wavelengths)
Endotoxin (LAL)
0.055 EU/mg
MFD / BBD
6/1/2026 / 6/1/2028
Storage on receipt
Room temperature
Traces reproduced
UV chromatogram (TWC) and mass spectrum
No link to a laboratory copy. This July 2026 certificate carries no QR code or other link
to a copy held by the laboratory, unlike the 1000 mg certificate.
Report date and Certificate ID don't
agree. The report date and the sample-received date both read August 24, 2026, as does the instrument data file
named above the UV trace (20260824). The Certificate ID reads 26Aug2026-02,
two days later, and the PDF itself was created on August 26. On most certificates in our library the ID begins with
the report date, and every one whose ID doesn't follows the same pattern: an August 2026 report addressed to Cell
Wellness, with an ID dated one or two days after the report date and a PDF created on the ID's date. The PDF doesn't
explain the difference.
The
laboratory's copy is identical. The QR code on both pages opens a copy of this certificate on AxisPharm's website.
On October 5, 2026 we compared that copy with the PDF published here: the two files were identical, byte for byte, so
the date difference is in the laboratory's own copy too.
No amount in the sample name. The sample name
reads “NAD+” with no amount, and there is no label-claim field; the 1000 mg size is
indicated by the lot number (NAD1000…) and the measured 1005.0 mg.
Reference identity data are cross-checked against PubChem and at least one further source.
On a certificate, an [M+H] ion is the molecule carrying one extra proton, so it reads at about the molecular
weight plus one; [M+2H] and [M+3H] carry two or three protons and read at about (MW + 2) ÷ 2
and (MW + 3) ÷ 3.
Lot
Ion
Calculated (average mass)
Detected
Difference
NAD500VAS0626
[M+H]
664.4
664.8
+0.4
NAD1000VAS0826
[M+H]
664.4
664.8
+0.4
Calculated on an average-mass basis: the molecular weight printed on each certificate plus 1.007 per proton, divided by the charge. An instrument that resolves isotopes reports the monoisotopic
peak instead, which sits lower: for NAD+ the monoisotopic [M+H] is 664.12, about 0.3 lower. The certificates don't state which basis the lab
reports or the instrument's mass accuracy, so the differences are shown, not graded.
Both certificates this guide covers report the same ion, 664.8 [M+H], 0.4 above the value calculated from the
printed 663.43 g/mol. NADH, the reduced form, is two hydrogens heavier (665.4 g/mol), so its [M+H] would
read near 666.4, 1.6 above the reported ion.
The reproduced mass spectra also label peaks that the results tables don't identify. Two of them, 348.3 (348.4 on
the 1000 mg certificate) and 428.6, are close to the [M+H] values of AMP (348.2) and ADP (428.2), smaller
molecules that make up part of the NAD+ structure. In the NAD500VAS0626 spectrum the tallest peak is 428.6, with 664.8
at about a third of its height; in the 1000 mg spectrum 664.8 is the tallest. The certificates don't say whether
those smaller ions formed in the instrument or were present in the sample.
What each test on the certificate means
UV purity
The lab separates the sample by liquid chromatography and records ultraviolet absorbance as it elutes.
UV purity is the target peak's share of the total peak area in the UV trace (at 208 nm where the
results table prints a wavelength). It counts only material that absorbs ultraviolet light: water, salts and the
counterion don't register, so it isn't the share of the powder that is NAD+.
Each certificate also states that salts and sugars present as fillers
aren't counted as impurities. A result written with “>”, such as “>99.3”,
is a lower bound, not an exact figure. A reproduced UV trace labelled XWC is extracted at the one
wavelength shown; one labelled TWC, a total-wavelength chromatogram, combines the detector's readings across its
wavelength range. More in
how peptide purity is measured and
how to read the HPLC chromatogram.
The main UV peak on both certificates this guide covers appears within the first half-minute of the
run (0.35 min). The certificates don't state the column, the
gradient or when unretained material comes off the column, so how well that peak was separated from other
UV-absorbing material can't be judged from the documents. The NAD500VAS0626 certificate's trace is labelled TWC, a
total-wavelength chromatogram, and the 1000 mg certificate's is extracted at 200.0 nm; neither results
table prints a wavelength.
Identity by LC-MS
The mass spectrometer reports ions by mass-to-charge ratio, and the certificate prints the molecular
weight next to the ion it detected. A detected ion close to the calculated value supports the identity of the
main component. It doesn't measure quantity, and molecules of the same mass (isomers) look alike to it.
Net weight measured
The lab's measured amount of material for the sample, printed beside the label claim where there is one.
The certificates don't say how it was measured, and it isn't net NAD+ content, which they don't report.
Endotoxin (LAL)
Bacterial endotoxin, the lipopolysaccharide of Gram-negative bacteria, measured with a Limulus amebocyte
lysate (LAL) assay and reported in endotoxin units per milligram of sample (EU/mg). The certificates print the
value only, with no acceptance limit and no LAL method variant. More in
endotoxin on a peptide COA.
MFD and BBD
Manufacture and best-before dates, printed in the sample description next to the lot number.
The certificates report no stability study, so a BBD is the date given for that lot, not a shelf life measured
under a stated storage condition.
Sample received and storage on receipt
When the lab received the sample, in what condition, and how the lab held it. That storage line is a
record of the lab's handling, not a storage recommendation.
Certificate ID and report date
The report's identifier and issue date. On most AxisPharm certificates in our library the ID begins with
the report date (13Jul2026-01 for a July 13, 2026 report, for example), which makes the two a useful
cross-check.
How to check one of these certificates
Match the lot. Find “Lot#” in the Sample Name line (NAD500VAS0626 on
that lot's certificate) and compare it with the lot number on the vial
label. A different lot number means a different certificate.
Check who issued it, and to whom. “From” names the laboratory; “To” names the
client the report went to.
Read the dates together. The report date, the date at the start of the Certificate ID, the
sample-received date and the MFD and BBD should tell one consistent story. Where they don't on the
certificates this guide goes through, the note on that certificate says so.
Compare the detected mass with the expected one. For an [M+H] ion, roughly the printed molecular
weight plus one. The identity table does this for each lot.
Read the purity with its method. A UV peak-area share, not the share of the powder that is
NAD+.
Note the endotoxin unit. EU/mg, endotoxin units per milligram of sample.
Open the laboratory's copy. Where a certificate carries a QR code, it links to a
copy on AxisPharm's website that can be compared with the PDF here, field by field.
Look for the traces. Two-page reports reproduce the UV chromatogram and the mass
spectrum.
Net NAD+ content: the share of the powder that is NAD+.
Counterion identity and amount.
Water content and residual solvents.
The column, gradient and mobile phase behind the UV purity, and, in the results tables, the wavelength.
A stability study: the BBD is a date given for the lot, not a measured shelf life.
Identities for the other peaks in the reproduced mass spectrum: the results tables name only the ions they report.
Which salt form was tested (free acid or a sodium salt). The printed 663.43 g/mol is the free acid's molecular weight.
Where these certificates come from, and caveats
Who ordered the tests. PepsMartUSA didn't. AxisPharm, LLC issued each certificate, and our
supplier, Protixa, provided it to us. The NAD+ 500mg certificate is addressed to Protixa and the NAD+ 1000mg certificate to Cell Wellness, a separate company under the same ownership as Protixa.
Each prints Protixa as the brand name. More in
where the certificates come from.
No second laboratory. No second laboratory's result is published for these lots.
As provided. We publish each PDF as our supplier provides it and don't edit it.
One lot each. A certificate describes the sample the lab tested from one lot. The lot number is the
only link between a vial and a certificate. The laboratory's footer on each page
says the same: its analytical data apply only to the batch tested.
The vials are lyophilized (freeze-dried) powder. The label's storage line reads −20 °C long term,
2–8 °C short term, protect from light: the freezer for long-term holding, a refrigerator for a short hold,
dark and dry in both cases, with the seal intact. The certificates describe the material on their report
dates; storage affects how it changes afterwards. Full detail in peptide
storage.
The NAD+ reference notes that solid NAD+ is hygroscopic (it takes up
moisture from the air) and light sensitive, so the seal and protection from light matter as much as the
temperature.
Questions about the NAD+ certificates this guide covers
Is the material on these certificates NAD+ or NADH?
The detected ion points to NAD+. NAD500VAS0626 and NAD1000VAS0826 both report 664.8 [M+H], 0.4 above the value calculated from
their printed molecular weight, 663.43 g/mol (C21H27N7O14P2,
PubChem CID 5892). NADH, the reduced form, is 665.4 g/mol, so its [M+H] would read near 666.4. The
certificates don't report NADH as a separate figure.
Do the certificates say which salt form of NAD+ was tested?
No. Both print 663.43 g/mol, the molecular weight of the free acid, and neither names a salt form or
reports sodium or water content. The detected ion doesn't settle it either: the [M+H] ion comes from the NAD+
molecule itself, so it doesn't show which counterion, if any, the solid contained. The
NAD+ reference explains why salt form and water content matter when comparing lots.
Why is the 1000 mg certificate's ID dated after its report?
Its report date and sample-received date read August 24, 2026, as does the instrument data file; its
Certificate ID, 26Aug2026-02, carries the date the PDF was created, August 26. The certificate
doesn't explain it. Every certificate in our library whose ID and report date differ is an August 2026 report
addressed to Cell Wellness with the same pattern. The QR code on this one opens a copy on AxisPharm's website that
was byte-for-byte identical to ours when we compared them on October 5, 2026, so the difference is in the
laboratory's own file.
Which vial does an NAD+ certificate describe?
Only the lot printed on it: NAD500VAS0626 or NAD500VAS0126 for a 500 mg certificate and NAD1000VAS0826 for the 1000 mg certificate. A certificate describes the sample the
laboratory tested from one lot; the lot number on a vial's label is what ties that vial to a certificate. The
lot lookup opens the certificate for a lot number.
Did PepsMartUSA order these tests?
No. AxisPharm, LLC issued them and our supplier, Protixa, provided them. The NAD500VAS0626 certificate is
addressed to Protixa and the NAD1000VAS0826 certificate to Cell Wellness, a separate company under the same ownership as Protixa; both print Protixa as the brand name. No
second laboratory's result is published for either lot.
For research use only. Not FDA approved, and not for
human or animal consumption. These certificates are laboratory documents about the material tested; nothing
on this page is an instruction for use of any kind. See the research use policy.
