Klotho Frag certificate of analysis, field by field
Updated 2026-10-05 · 1 certificate ·
Issued by AxisPharm, LLC · Laboratory documentation · Research use only
PepsMartUSA publishes one certificate of analysis for Klotho Frag, covering the 10 mg vial.
It was issued by AxisPharm, LLC, the testing laboratory our supplier, Protixa, sends samples to, and Protixa provided
it to us. Below: every field the certificate prints, what each test measures and what it doesn't report, with one limit
stated up front: neither the product name nor the certificate gives the peptide's sequence, so its detected mass can
be compared only with the molecular weight the certificate itself prints.
Issued by AxisPharm, LLC, provided by our supplier, Protixa. Identity for every lot was by LC-MS;
the detected masses are under identity below.
The certificate, field by field
The fields below are copied from the PDF as printed, including any that disagree with each other.
Klotho Frag 10mg · Lot KLO10VAS0626
Laboratory (From)
AxisPharm, LLC
Addressed to (To)
Protixa
Brand name
Protixa
Certificate ID
09Jul2026-01
Report date
July 09, 2026
Sample received
07/07/2026
Condition on receipt
Acceptable
Sample name
Klotho Frag, 10MG
Method(s)
LCMS and LAL Endotoxin Assay
Net weight measured
10.2 mg
Molecular weight
1152.5 g/mol
Detected
1153.0 [M+H]
UV purity
>99.3% at 208 nm
Endotoxin (LAL)
0.057 EU/mg
MFD / BBD
06/2026 / 06/2028
Storage on receipt
Room temperature
Traces reproduced
UV chromatogram (TWC) and mass spectrum
Lot number in a different typeface. The lot number, KLO10VAS0626, is
printed in a sans-serif typeface, unlike the text around it, as on other AxisPharm reports in our library from
April to June 2026. The PDF's own data record one creation, by Microsoft Print to PDF on July 9, 2026, the report
date, and no later revision, so the lot number was printed that way when the file was made.
We don't publish reference identity data for Klotho Frag. “Klotho Frag” is our
supplier's product name, and neither it nor the certificate gives a sequence, a molecular formula or a CAS number,
so there is no published structure to check the printed 1152.5 g/mol against. What the certificate does show is
that its own figures agree: the detected 1153.0 [M+H] is 0.5 below the value calculated from its printed molecular
weight.
On a certificate, an [M+H] ion is the molecule carrying one extra proton, so it reads at about the molecular
weight plus one; [M+2H] and [M+3H] carry two or three protons and read at about (MW + 2) ÷ 2
and (MW + 3) ÷ 3.
Lot
Ion
Calculated (average mass)
Detected
Difference
KLO10VAS0626
[M+H]
1153.5
1153.0
−0.5
Calculated on an average-mass basis: the molecular weight printed on the certificate plus 1.007 per proton, divided by the charge. The certificate doesn't state which basis the lab
reports or the instrument's mass accuracy, so the differences are shown, not graded.
What each test on the certificate means
UV purity
The lab separates the sample by liquid chromatography and records ultraviolet absorbance as it elutes.
UV purity is the target peak's share of the total peak area in the UV trace (at 208 nm where the
results table prints a wavelength). It counts only material that absorbs ultraviolet light: water, salts and the
counterion don't register, so it isn't the share of the powder that is peptide.
The certificate also states that salts and sugars present as fillers
aren't counted as impurities. A result written with “>”, such as “>99.3”,
is a lower bound, not an exact figure. A reproduced UV trace labelled XWC is extracted at the one
wavelength shown; one labelled TWC, a total-wavelength chromatogram, combines the detector's readings across its
wavelength range. More in
how peptide purity is measured and
how to read the HPLC chromatogram.
The results table gives UV purity at 208 nm, but the reproduced UV trace is
labelled TWC, a total-wavelength chromatogram, rather than a 208 nm trace, so the printed figure can't be read
directly off the trace. The main peak appears at 1.94 min.
Identity by LC-MS
The mass spectrometer reports ions by mass-to-charge ratio, and the certificate prints the molecular
weight next to the ion it detected. A detected ion close to the calculated value supports the identity of the
main component. It doesn't measure quantity, and molecules of the same mass (isomers) look alike to it.
Net weight measured
The lab's measured amount of material for the sample, printed beside the label claim where there is one.
The certificate doesn't say how it was measured, and it isn't net peptide content, which it doesn't report.
Endotoxin (LAL)
Bacterial endotoxin, the lipopolysaccharide of Gram-negative bacteria, measured with a Limulus amebocyte
lysate (LAL) assay and reported in endotoxin units per milligram of sample (EU/mg). The certificate prints the
value only, with no acceptance limit and no LAL method variant. More in
endotoxin on a peptide COA.
MFD and BBD
Manufacture and best-before dates, printed in the sample description next to the lot number.
The certificate reports no stability study, so a BBD is the date given for that lot, not a shelf life measured
under a stated storage condition.
Sample received and storage on receipt
When the lab received the sample, in what condition, and how the lab held it. That storage line is a
record of the lab's handling, not a storage recommendation.
Certificate ID and report date
The report's identifier and issue date. On most AxisPharm certificates in our library the ID begins with
the report date (13Jul2026-01 for a July 13, 2026 report, for example), which makes the two a useful
cross-check.
How to check this certificate
Match the lot. Find “Lot#” in the Sample Name line (KLO10VAS0626 on
that lot's certificate) and compare it with the lot number on the vial
label. A different lot number means a different certificate.
Check who issued it, and to whom. “From” names the laboratory; “To” names the
client the report went to.
Read the dates together. The report date, the date at the start of the Certificate ID, the
sample-received date and the MFD and BBD should tell one consistent story. Where they don't on the
certificates this guide goes through, the note on that certificate says so.
Compare the detected mass with the expected one. For an [M+H] ion, roughly the printed molecular
weight plus one. The identity table does this for each lot.
Read the purity with its method. A UV peak-area share, not the share of the powder that is
peptide.
Note the endotoxin unit. EU/mg, endotoxin units per milligram of sample.
Look for the traces. Two-page reports reproduce the UV chromatogram and the mass
spectrum.
Net peptide content: the share of the powder that is peptide.
Counterion identity and amount (trifluoroacetate or acetate, for example).
Water content and residual solvents.
The column, gradient and mobile phase behind the UV purity.
A stability study: the BBD is a date given for the lot, not a measured shelf life.
Identities for the other peaks in the reproduced mass spectrum: the results table names only the ion it reports.
Where this certificate comes from, and caveats
Who ordered the tests. PepsMartUSA didn't. AxisPharm, LLC issued the certificate, and our
supplier, Protixa, provided it to us. It is addressed to Protixa.
It prints Protixa as the brand name. More in
where the certificates come from.
No second laboratory. No second laboratory's result is published for this lot.
As provided. We publish each PDF as our supplier provides it and don't edit it.
One lot each. A certificate describes the sample the lab tested from one lot. The lot number is the
only link between a vial and a certificate. The laboratory's footer on each page
says the same: its analytical data apply only to the batch tested.
The vials are lyophilized (freeze-dried) powder. The label's storage line reads −20 °C long term,
2–8 °C short term, protect from light: the freezer for long-term holding, a refrigerator for a short hold,
dark and dry in both cases, with the seal intact. The certificate describes the material on its report date; storage affects how it changes afterwards. Full detail in peptide
storage.
The certificate doesn't say. Its sample name reads “Klotho Frag, 10MG”, and it prints a
molecular weight, 1152.5 g/mol, but no sequence, formula or CAS number. Our supplier's product name doesn't
identify the sequence either, so we don't publish one.
What does the Klotho Frag certificate show about identity?
That the main component has the mass the laboratory expected: 1153.0 [M+H] detected, against about 1153.5
calculated from the printed 1152.5 g/mol, 0.5 apart. Without a sequence or formula, the reading can't be
compared with a published reference structure.
Which vial does the Klotho Frag certificate describe?
Only the lot printed on it, KLO10VAS0626. A certificate describes the sample the
laboratory tested from one lot; the lot number on a vial's label is what ties that vial to a certificate. The
lot lookup opens the certificate for a lot number.
Did PepsMartUSA order this test?
No. AxisPharm, LLC issued the certificate and our supplier, Protixa, provided it.
It is addressed to Protixa and prints Protixa as the brand name. No second laboratory's result is published for
this lot.
For research use only. Not FDA approved, and not for
human or animal consumption. This certificate is a laboratory document about the material tested; nothing
on this page is an instruction for use of any kind. See the research use policy.
